A9

85011426, mouse adipose tissue, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: A9, 85011426
• 생물학적 소스: mouse adipose tissue
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C3H/An areolar and adipose tissue

세포주 설명

Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

애플리케이션

Metabolic studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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