MDCK Cell Line canine

84121903, canine kidney, Epithelial

• 동의어(들): MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSC 코드: 41106514
• eCl@ss: 32190102

속성

• 제품명: MDCK Cell Line canine, 84121903
• 생물학적 소스: canine kidney
• 양식: cell suspension
• 성장 모드: Adherent
• 핵형: 2n = 78
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Canine Cocker Spaniel kidney

세포주 설명

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

애플리케이션

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

기타 정보

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