MRC-5 SV2

84100401, human lung (fetal), Fibroblast

• 동의어(들): MRC-5V2 Cells, MRC5V2 Cells
• UNSPSC 코드: 41106514

속성

• product name: MRC-5 SV2, 84100401
• 생물학적 소스: human lung (fetal)
• 성장 모드: Adherent
• 핵형: Modal no. = 60-80, hyperdiploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human foetal lung, SV40 transformed

세포주 설명

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

애플리케이션

Transformation and virus studies: SV40

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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