OX-115

13082229, from Rat x Rat Hybridoma

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• 골수종: Y3
• 반응성: Recombinant CD147 domain-0
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 설명

The hybridoma cell line OX-115 recognises an alternatively spliced domain of mouse CD147.

면역원

Recombinant CD147

애플리케이션

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

계대배양 정규 작업

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

기타 정보

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.