AB-1 HA

12070506

• 동의어(들): AB 1 HA, AB-1HA
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse mesothelium
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
• 수용체: Not specified
• 관련 질환(들): cancer

설명

세포주 기원

Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)

세포주 설명

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.

DNA 프로파일

Not specified

배양 배지

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442) + 400ug/ml G418.

계대배양 정규 작업

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.

기타 정보

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia