AB1 cell line from mouse lung

10092305, mouse lung. Fibroblast-like, but demonstrates typical epithelial cobblestone morphology

• UNSPSC 코드: 41106514

속성

• product name: AB1 cell line from mouse lung, 10092305
• 생물학적 소스: mouse lung
• 설명: Mouse malignant mesothelioma
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast-like but shows a typical epithelial "cobblestone"
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse malignant mesothelioma

세포주 설명

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0144

애플리케이션

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

배양 배지

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

계대배양 정규 작업

Optimal split ratio 1:8 (seeding density 1.2 x104 cells/cm²).Cells incubated at 37oC with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

기타 정보

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake, University of Western Australia, Australia