LIM1215 human

10092301, human colon, Epithelial

• 동의어(들): LIM1215 Cell Line
• UNSPSC 코드: 41106514

속성

• 제품명: LIM1215 human, 10092301
• 생물학적 소스: human colon
• 설명: Colon carcinoma from human colon
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Colorectal carcinoma from human colon

Human colorectal carcinoma

애플리케이션

For complete product information, please see ECACC

배양 배지

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6?g/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM

계대배양 정규 작업

Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

기타 정보

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Tony Burgess - Ludwig Institute for Cancer Research, Australia