MKL-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.

• 동의어(들): MKL1 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human skin
• 포장: tube of 5 μg 09111801-DNA-5UG; pkg of vial of cells 09111801-1VL
• 성장 모드: Suspension
• 핵형: Hyperdiploid
• 형태학: Loosely packed floating aggregates with irregular outline and no central necrosis
• 제품: MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice

설명

세포주 기원

Human neuroendocrine skin carcinoma

세포주 설명

MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).

DNA 프로파일

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18

배양 배지

RPMI 1640 + 2 mM Glutamine + 10% FBS

계대배양 정규 작업

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37 °C Saturation density is approximately 10⁶ cells/ml. Population doubling time is around 120 hours.

기타 정보

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