Mero-14

09100101

• 동의어(들): Mero14 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human lung
• 성장 모드: Adherent
• 핵형: Number of chromosomes 72-75
• 형태학: Spindle shaped cells
• 제품: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

세포주 설명

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

애플리케이션

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

배양 배지

Hams F10 + 15% FCS + 2mM Glutamine

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

기타 정보

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