328/14

8062541, mouse nerve (fibroblast), Neuronal/fibroblastic

• UNSPSC 코드: 41106514

속성

• product name: 328/14, 08062541
• 생물학적 소스: mouse nerve (fibroblast)
• 성장 모드: Adherent
• 핵형: Modal No. varied with passage see reference
• 형태학: Neuronal/fibroblastic
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse neuroblastoma x mouse L cell fibroblast hybrid

세포주 설명

Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (Sigma Catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (Sigma Catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/14 is of the neuronal type.

애플리케이션

Used as a neuronal Model

배양 배지

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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