C6-4-2

8062524, rat brain, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: C6-4-2, 08062524
• 생물학적 소스: rat brain
• 포장: tube of 5 μg 08062524-DNA-5UG; pkg of vial of cells 08062524-1VL
• 성장 모드: Adherent
• 핵형: modal No varied with passage
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat glioma x rat glioma hybrid

세포주 설명

Pseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant mutant rat octaploid glioma cell line C6-3-BU-8 and the 6-thioguanine-resistant mutant rat octaploid glioma cell line C6-3-TG-3. C6-4-2 cells provide a suitable model system for studying the regulation of the membrane potential by peptide hormones.

애플리케이션

electrophysiology

배양 배지

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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