PNT2 (SERUM FREE)

7042701, human prostate, Epithelial

• UNSPSC 코드: 41106514
• 생물학적 소스: human prostate
• 성장 모드: Adherent
• 핵형: Not
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified

속성

• 제품명: PNT2 (SERUM FREE), 07042701
• 생물학적 소스: human prostate
• 성장 모드: Adherent
• 핵형: Not
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human prostate normal, serum-free

세포주 설명

This cell line is a derivative of the normal human prostate cell line PNT2 (Sigma cat no. 95012613) adapted to grow in serum-free medium. PNT2 was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein.

배양 배지

Defined Keratinocyte-SFM (Gibco 10785) + Growth Supplement (Gibco 10784-015).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Use Trypsin Inhibitor to inactivate the trypsin. When freezing cells down use conditioned medium plus 10% DMSO.

기타 정보

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