KARPAS-25

6072601, human lung (effusion), Round to polygonal cells growing singly in suspension

• 동의어(들): KARPAS 25, KARPAS, 25, KARPAS25
• UNSPSC 코드: 41106514

속성

• 제품명: KARPAS-25, 06072601
• 생물학적 소스: human lung (effusion)
• 성장 모드: Suspension
• 핵형: Hyperdiploid with complex aberrant chromosomes
• 형태학: Round to polygonal cells growing singly in suspension
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice

설명

세포주 기원

Human acute plasma cell leukaemia

세포주 설명

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

배양 배지

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

계대배양 정규 작업

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

기타 정보

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면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.