MRC-5 pd25 Cell Line human

5081101, human lung, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• 제품명: MRC-5 pd25 Cell Line human, fetal lung, 05081101
• 생물학적 소스: human lung
• 성장 모드: Adherent
• 핵형: 2n=46, diploid
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human foetal lung

세포주 설명

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

애플리케이션

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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