MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• 동의어(들): MDCK-SIAT1 Cells
• UNSPSC 코드: 41106514

속성

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• 생물학적 소스: canine kidney
• 설명: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Enhanced expression of 6-linked sialic acids
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

세포주 설명

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

배양 배지

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.