P3X63Ag8.653-TurboDoma

3092502, mouse blood, Lymphoblast

• UNSPSC 코드: 41106514

속성

• product name: P3X63Ag8.653-TurboDoma, 03092502
• 생물학적 소스: mouse blood
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/c myeloma, serum-free

세포주 설명

P3X63Ag8.653 cell line (Sigma Catalogue number. 85011420) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma^™. Suitable for use as a fusion parent for serum-free generation of hybridomas.

애플리케이션

Fusion partner for generation of serum-free hybridomas.

배양 배지

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

계대배양 정규 작업

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

기타 정보

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법적 정보

TurboDoma is a trademark of Cell Culture Technologies