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Merck
모든 사진(3)

주요 문서

C5499

Sigma-Aldrich

Cytochrome c Oxidase from bovine heart

greener alternative

5 mg protein/mL

동의어(들):

EC 1.9.3.1, Ferrocytochrome-c:oxygen oxidoreductase, Complex IV

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32

생물학적 소스

bovine heart

Quality Level

양식

buffered aqueous solution

특이 활성도

≥20 units/mg protein

분자량

300-350 kDa by gel filtration

환경친화적 대안 제품 특성

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

농도

5 mg protein/mL

환경친화적 대안 카테고리

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Research area: Cell cycle

Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

애플리케이션

Cytochrome c Oxidase (CcO) from bovine heart has been used:
  • to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines
  • in a histochemical assay to measure CcO activity in tissue sections
  • to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

생화학적/생리학적 작용

Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation.

단위 정의

One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0.

물리적 형태

Supplied as a solution in 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl β·-D-maltoside.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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