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Key Documents

C-39606

Sigma-Aldrich

Renal Epithelial Cell Growth Medium 2

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

동의어(들):

Recommended with C-26230

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

형태

aqueous solution

포장

pkg of 1 vial

기술

cell culture | mammalian: suitable

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Renal Epithelial Cell Growth Medium 2 SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-26230

애플리케이션

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

성분

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

품질

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

제조 메모

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 36 ng / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 4 pg / mlTransferrin (recombinant human): 5 µg / ml

저장 및 안정성

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

계대배양 정규 작업

Click here for more information.

추천

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

문서

Primary human renal epithelial cells and low-serum growth medium from PromoCell are optimized for the in vitro cultivation of epithelial cells from the kidney. Protocols for handling, cell passaging, media, and product use.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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