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Merck
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주요 문서

C-28030

Sigma-Aldrich

Mononuclear Cell Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.81

양식

liquid

포장

pkg of 500 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

일반 설명

Mononuclear Cell Medium (Ready-to-use)

애플리케이션

Mononuclear Cell Medium is a very rich short-term maintenance medium, which provides utmost cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood and other sources, e.g. bone marrow. It is suitable to maintain Mononuclear Cells for up to 48 hours before proceeding with your experiments.Recommended for:Human Mononuclear Cells from Cord Blood (hMNC-CB)Human Mononuclear Cells from Peripheral Blood (hMNC-PB)

품질

All lots of PromoCell Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of PromoCell Mononuclear Cell Medium is checked for unaltered shortterm maintenance of highly viable mononuclear cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

제조 메모

The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium.

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix is delivered thawed and can be frozen after arrival without losing any activity

계대배양 정규 작업

Click here for more information.

Storage Class Code

10 - Combustible liquids

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

문서

Primary Human Mononuclear Cells and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Hematopoietic Stem and Immune Cell Culture

Cryopreserved blood cells and cell culture media for CD34+ human hematopoietic progenitor stem cells, monocytes, PBMCs, macrophages, dendritic and T-cells. Explore over 350 PromoCell products on Sigma.com.

프로토콜

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products on Sigma.com.

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Promocell

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

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