C2780

Chondroitinase AC from Flavobacterium heparinum

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

• 동의어(들): Chondroitin AC lyase
• CAS Number: 9047-57-8
• 효소 위원회 번호: 4.2.2.5 (BRENDA, IUBMB)
• MDL number: MFCD00130796
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 결합: (Glucosaminoglycan)
• Quality Level: 200
• 형태: lyophilized powder
• 특이 활성도: 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
• 구성: Protein, ~15% Lowry
• solubility: 0.02 M phosphate buffer: soluble (pH 7.0)
• 외래 활성: Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
• 저장 온도: −20°C

설명

애플리케이션

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

생화학적/생리학적 작용

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

기타 정보

Contains potassium phosphate buffer salts and BSA as stabilizer.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

적합성

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

단위 정의

One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

제조 메모

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)