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Merck
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Key Documents

C-23060

Sigma-Aldrich

Skeletal Muscle Cell Growth Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

동의어(들):

Primary Cell Culture Media

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.81

형태

liquid

포장

pkg of 500 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

일반 설명

Skeletal Muscle Cell Growth Medium (Ready-to-use)

애플리케이션

Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

성분

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

품질

All lots of PromoCell Skeletal Muscle Cell Growth Medium are subjected to comprehensive quality control tests using human primary SkMC. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

제조 메모

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlFetuin (bovine): 50 µg / mlEpidermal Growth Factor (recombinant human): 10 ng / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 10 µg / mlDexamethasone: 0.4 µg / ml

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

계대배양 정규 작업

Click here for more information.

Storage Class Code

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

문서

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

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