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Merck
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Key Documents

C-12907

Sigma-Aldrich

Human Mononuclear Cells (hMNC)

Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

동의어(들):

hMNC cells

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About This Item

UNSPSC 코드:
41106514
NACRES:
NA.81

생물학적 소스

human peripheral blood

포장

pkg of 25,000,000 cells

형태학

(mononuclear)

기술

cell culture | mammalian: suitable

배송 상태

dry ice

저장 온도

−196°C

일반 설명

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

세포주 기원

Blood

애플리케이션

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

품질

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

경고

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

계대배양 정규 작업

Click here for more information.

기타 정보

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

문서

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Cryopreserved blood cells and cell culture media for CD34+ human hematopoietic progenitor stem cells, monocytes, PBMCs, macrophages, dendritic and T-cells. Explore over 350 PromoCell products on Sigma.com.

프로토콜

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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