추천 제품
양식
liquid
농도
1% in 3% acetic acid, pH 2.5
기술
microbe id | staining: suitable
색상
dark blue to very dark blue
pH 범위
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
애플리케이션
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
생화학적/생리학적 작용
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)
Mario Barilani et al.
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