B1310

Anti-BACH1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-BRCA1-associated C-terminal helicase 1
• MDL number: MFCD06201924
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~150 kDa
• 종 반응성: human
• 기술: immunoprecipitation (IP): 5-10 μg using RIPA extract (0.5 mg) of human HeLa cells; indirect immunofluorescence: 10-20 μg/mL using human MCF-7 cells; microarray: suitable; western blot: 0.5-1.0 μg/mL using whole extracts of MCF-7 human breast carcinoma cells
• UniProt 수납 번호: Q9BX63
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... BRIP1(83990) ; mouse ... Brip1(237911) ; rat ... Brip1(360588)

설명

일반 설명

BTB Domain And CNC Homolog 1 (BACH1) is expressed in numerous tissues. The protein includes a nuclear localization signal in its helical domain.

면역원

synthetic peptide corresponding to amino acid residues 1233-1249 of human BACH1 with N-terminal added cysteine, conjugated to KLH.

애플리케이션

Anti-BACH1 antibody produced in rabbit has been used in:

• immunoblotting
• immunohistochemistry
• western blotting

Polyclonal anti-BACH1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10 μg by using RIPA extract (0.5 mg) of human HeLa cells and for indirect immunofluorescence at 10-20 μg/mL using human MCF-7 cells. The product is also suitable for western blot analysis at a working concentration of 0.5-1.0 μg/mL by using whole extracts of MCF-7 human breast carcinoma cells. Further, anti-BACH1 antibody has shown suitability for microarray analysis.

생화학적/생리학적 작용

BACH1 interacts directly with the C-terminal BCRT repeats in Breast cancer type1 protein (BRCA1), a tumor suppressor nuclear phosphoprotein that facilitates the repair of double-stranded DNA breaks. Bach1 also functions as transcription repressor in transfection assays using fibroblast cells and as a transcriptional activator and repressor, respectively, in cultured erythroid cells. Additionally, it is also involved in the coordination of transcription activation and repression by Maf family members such as MafK. Bach1 (BTB and CNC homology 1, basic leucine zipper transcription factor 1) inhibits oxidative stress-inducible genes and is a negative regulator of oxidative stress-induced cellular senescence.

BTB Domain And CNC Homolog 1 (BACH1) interaction between breast cancer type 1 (BRCA1) in vivo appears to be dependent on phosphorylation of Ser990 in the latter during the G2/M phase of the cell cycle. BACH1 mutations were found in patients with early-onset breast cancer. It displays a punctate nuclear staining pattern similar to that observed for BRCA1.

표적 설명

BACH1, BRCA1 interacting protein C-terminal helicase 1, encodes a protein that is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand br

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)