B0184

Bacteriorhodopsin from Halobacterium salinarum

native sequence, lyophilized powder

• 동의어(들): BR from H. salinarum, Bacterioopsin, Bacteriorhodopsin from Halobacterium halobium
• CAS Number: 53026-44-1
• MDL number: MFCD00130599
• UNSPSC 코드: 12352202
• NACRES: NA.56

속성

• 생물학적 소스: Halobacterium salinarium
• Quality Level: 100
• 양식: lyophilized powder
• 기술: ligand binding assay: suitable; mass spectrometry (MS): suitable
• UniProt 수납 번호: B0R5N9
• 저장 온도: 2-8°C
• 유전자 정보: Halobacterium salinarium ... OE_RS05715(5953595) , VNG_RS05715(144807)

설명

일반 설명

Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins.

Bacteriorhodopsin is the prototypical "seven-helix" transmembrane protein (with seven α-helical domains), whose study led to advances in understanding G protein-coupled receptors (GPCRs). In Halobacteria, it acts as a light-harvesting protein, producing a proton gradient across the cell wall that is then used to drive biosynthetic processes.

애플리케이션

Bacteriorhodopsin from Halobacterium salinarum has been used:

• in generation of droplet lipid bilayer
• as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS)
• in the generation of protein-detergent complex and micelles for dynamic light scattering studies

Bacteriorhodopsin is of interest in the development of artificial retinas, optical associative processors, and three-dimensional memory storage devices.

생화학적/생리학적 작용

Bacteriorhodopsin (BR) from Halobacterium salinarum acts as a proton-driven pump. BR can be used in studies of the folding and kinetics of α-helical proteins. It is thermally stable and exhibits high photoelectric and photochemical efficiency. BR exists as trimer in a hexagonal lattice. Its photocycle intermediates are exploited in bioelectronics majorly in photoelectric and photochemical applications.

A transmembrane retinylidine protein that functions as a proton pump driven by light energy in Holobacterium.

제조 메모

Aqueous suspensions may be sonicated to achieve the desired homogeneity and may be stored for a short time at a temperature of 2-8 °C or at a temperature of -20 °C without time limitation.

Wild-type bacteriorhodopsin is isolated from Halobacterium salinarum strain S9 as purple membranes.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)