A8062
Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal
clone GT-34, purified from hybridoma cell culture
동의어(들):
Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse
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모든 사진(3)
About This Item
UNSPSC 코드:
12352203
NACRES:
NA.46
추천 제품
생물학적 소스
mouse
결합
alkaline phosphatase conjugate
항체 형태
purified from hybridoma cell culture
항체 생산 유형
secondary antibodies
클론
GT-34, monoclonal
양식
buffered aqueous glycerol solution
종 반응성
sheep, goat, bovine
반응하면 안 됨
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
기술
direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
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관련 카테고리
일반 설명
IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
면역원
Purified goat IgG
애플리케이션
Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).
The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.
물리적 형태
Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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