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Merck
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Key Documents

A3316

Sigma-Aldrich

Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat

affinity isolated antibody, PBS suspension

동의어(들):

Goat anti-human IgG

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

agarose conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

형태

PBS suspension

종 반응성

human

반응하면 안 됨

rat, mouse

기술

Ouchterlony double diffusion: suitable

용량

2 mg/mL, resin binding capacity (human IgG)

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

면역원

Fc fragment of human IgG.

애플리케이션

Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.

기타 정보

Antibody adsorbed with mouse and rat IgG

물리적 형태

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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