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Merck
모든 사진(1)

주요 문서

A3275

Sigma-Aldrich

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

동의어(들):

Goat Anti-Human IgM (μ-chain specific)−AP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

alkaline phosphatase conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

양식

buffered aqueous glycerol solution

종 반응성

human

기술

direct ELISA: 1:7,000-1:21,000

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

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일반 설명

IgM antibody is produced by B cells and has pentamer structure which helps the antibody in polyreactivity and can also remove apoptotic cells. Anti-human IgM (μ-chain specific) -alkaline phosphatase antibody can be used in solid phase ELISA for determination of anti-sp75 antibodies. Goat anti human IgM-alkaline phosphatase antibody reacts specifically with human IgM.

면역원

Human IgM.

애플리케이션

IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma). Arnold, J. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin. JBC, 280: 29080-29087.
It may be used for immunoblotting.

물리적 형태

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Tzer Chyn Lim et al.
Current biology : CB, 28(6), 955-962 (2018-03-06)
The position of the division site dictates the size and fate of daughter cells in many organisms. In animal cells, division-site placement involves overlapping mechanisms, including signaling from the central spindle microtubules, astral microtubules, and spindle poles and through polar
Immunoglobulin M Reactivity Towards the Immunologicdly Active Region sp75 of the Core Protein of Hepatitis C Virus (HCV) in Chronic HCV Infection.
U.B. Hellstrorn, S.P.E. Sylvan
Journal of Medical Virology, 39(4), 39325-39332 (1993)
Francesca Gori et al.
Journal of neuroimmunology, 233(1-2), 216-220 (2011-01-11)
Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli
H A Cubie et al.
Journal of clinical pathology, 46(9), 840-845 (1993-09-01)
AIMS--To compare the sensitivity, specificity, and practicality of recombinant proteins in serological tests for the detection of human parvovirus B19 IgG and IgM. METHODS--Indirect enzyme linked immunosorbent assays using B19 structural proteins expressed in Escherichia coli were developed for the
Young Chan Kim et al.
Viruses, 11(5) (2019-05-06)
Chikungunya fever is a debilitating disease caused by Chikungunya virus (CHIKV) that can result in long-lasting arthralgias. The early diagnosis of CHIKV relies on PCR during the acute infection phase to allow differential diagnosis with other co-circulating arboviruses such as

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