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A25120

Sigma-Aldrich

DEAE–Sephadex® A-25 chloride form

동의어(들):

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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About This Item

CAS Number:
12609-80-2
MDL number:
MFCD00132778
UNSPSC 코드:
47101511
NACRES:
NA.56

양식

powder

Quality Level

200

기술

activity assay: suitable

Matrix

cross-linked dextran

기질 활성군

functional group

비드 크기

40-125 μm (dry)

공극 크기

~30,000 Da exclusion limit

작동 pH

2-9

용량

3-4 meq/g ion exchange capacity

호환성

mode of use mixed weak and strong anion exchange chromatography

일반 설명

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

애플리케이션

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

특징 및 장점

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

법적 정보

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000274266
SLCN0054
SLCN0054
0000274266
SLCM3767

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In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
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Experimental cell research, 139(2), 401-404 (1982-06-01)
H Wang et al.
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
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Epitopes for monoclonal antibodies directed against the purified adult rat skeletal muscle sodium channel (rSkM1) were localized using channel proteolysis and fusion proteins. The interactions between these and other monoclonal antibodies with site-specific polyclonal antibodies were used to investigate the
Isolation and study of insulin activated nitric oxide synthase inhibitory protein in acute myocardial infarction subjects.
Ray, U., et al.
Journal of Thrombosis and Haemostasis, 33(3), 218-229 (2012)
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
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