A0729

Monoclonal Anti-Activin B antibody produced in mouse

clone 146807, purified immunoglobulin

• MDL number: MFCD06798692
• UNSPSC 코드: 51111800
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 146807, monoclonal
• 종 반응성: human
• 기술: neutralization: 0.6-3.0 μg/mL using Activin B-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (ND₅₀); western blot: 1-2 μg/mL
• 동형: IgG1
• UniProt 수납 번호: P09529
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... INHBB(3625)

설명

일반 설명

Activins are members of the TGF-β family. Activins are dimeric proteins composed of 2 β subunits. Acitivn B contains 2 β B subunits. Activins bind to the Type II Activin receptor triggering phosphorylation of Type I Acitivn receptor (ALK4) and activation of downstream pathways such as proliferation and apoptosis.

Activins are pleotropic cytokines that belong to the TGF-β superfamily. There are three forms of activin, homodimeric activin A (βA-βA), activin B (βB-βB) and the heterodimeric activin AB (βA-βB). Activins bind to type I (ActRI) or type II (ActRII) receptor complexes. The cellular targets of Activin are Smad-2, -3 and -4 that form hetromeric complex and regulate transcription of target genes. The other pathways mediated by activins are Rho A, MAPK, p38 and JNK pathways. Activins play vital role in regulation of growth, differentiation, erythropoiesis, angiogenesis, survival or neural cells and tissue repair. Activin B is reported to promote wound healing by epithelial cell proliferation and migration via the RhoA-JNK signaling pathway. It is also vital in follicular development during the estrous cycle and normal fertility in female mammals. Various reports indicate the role of activin B in normal function of pancreatic β cells and in reducing the growth of renal cell carcinoma
Monoclonal Anti-Activin B recognizes human activin B.

면역원

purified, CHO cell-derived, recombinant human activin B.

애플리케이션

Anti-Activin B antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The recommended working antibody concentration for ELISA is 0.5-1.0 μg/ml. For neutralization of human activin B expressed in K562 cell line, a concentration of 0.6-3.0 μg/ml is recommended.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)