94581

Hirudin recombinant from yeast

lyophilized powder, white, >1500 U/vial

• CAS Number: 8001-27-2
• EC Number: 232-279-1
• MDL number: MFCD00803952
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: synthetic; yeast (Pichia pastoris)
• 양식: lyophilized powder
• 특이 활성도: >1500 U/vial
• 분자량: M_r ~6963.5
• 색상: white
• solubility: 0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless
• 저장 온도: −20°C

설명

애플리케이션

Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay.

생화학적/생리학적 작용

Recombinant hirudin appears to be antigenic. Independent studies have found that 50-70% of patients treated for several days with recombinant hirudin develop at least one hirudin antibody isotype. The antibodies reduce the anticoagulant effect of hirudin, but prolong its plasma half-life by reducing renal filtration.

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

단위 정의

1 U corresponds to 1 ATU (antithrombin unit; 1 ATU is defined as the amount of inhibitor that neutralizes 1 NIH unit of thrombin).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)