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Merck
모든 사진(1)

Key Documents

86090201

HeLa229 cell line

from human cervix

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
41106514

생물학적 소스

human cervix

Quality Level

형태

liquid

성장 모드

Adherent

핵형

2n = 46

형태학

Epithelial

제품

Not specified

수용체

Not specified

기술

cell culture | mammalian: suitable

관련 질환(들)

cancer

배송 상태

dry ice

저장 온도

−196°C

세포주 기원

Human cervix carcinoma

세포주 설명

Derived from the parent HeLa line but differs chiefly in its relative insusceptibility to polioviruses. The presence of human papilloma virus 18 (HPV-18) sequences in HeLa cells has been reported.

애플리케이션

Tumourigenicity and virus studies, poliovirus 1 and AD3

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

배양 배지

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Luis Crovetto et al.
The journal of physical chemistry. A, 119(44), 10854-10862 (2015-10-16)
Dyes with near-red emission are of great interest because of their undoubted advantages for use as probes in living cells. In-depth knowledge of their photophysics is essential for employment of such dyes. In this article, the photophysical behavior of a
C Yee et al.
The American journal of pathology, 119(3), 361-366 (1985-06-01)
A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16, and HPV-18 DNA probes. Six of eight cell lines derived from human cervical carcinomas were shown to contain

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