51456

Urea

BioUltra, for molecular biology, 99% (T)

• 동의어(들): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC 코드: 12352111
• PubChem Substance ID: 57651307
• NACRES: NA.25

속성

• Grade: for molecular biology
• 제품 라인: BioUltra
• 분석: 99% (T)
• 양식: powder or crystals
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.1% biuret; <0.005% formaldehyde (HCHO)
• 무기 잔류물: ≤0.01% (as SO₄)
• 손실: ≤0.1% loss on drying, 20 °C (HV)
• pH: 8.0-10.0 (20 °C, 8 M in H₂O)
• mp: 132-135 °C (lit.)
• solubility: H₂O: 8 M at 20 °C, clear, colorless
• density: 1.335 g/mL at 25 °C (lit.)
• 음이온 미량물: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: NC(N)=O
• λ: 8 M in H₂O
• UV 흡수: λ: 260 nm A_max: ≤0.03; λ: 280 nm A_max: ≤0.02
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• 유전자 정보: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

설명

애플리케이션

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

기타 정보

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)