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Merck
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Key Documents

18373

Sigma-Aldrich

Atto 647N NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL number:
UNSPSC 코드:
12352108
NACRES:
NA.32

제품 라인

BioReagent

Quality Level

분석

≥90% (HPLC)
≥90% (degree of coupling)

형태

solid

제조업체/상표

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV 흡수

λ: 641-647 nm Amax

적합성

suitable for fluorescence

저장 온도

−20°C

일반 설명

Atto 647N is a superior red-emitting fluorescence dye with a strong absorption, excellent fluorescence quantum yield (65%), high photostability, excellent ozone resistance, good solubility, and very little triplet formation.

애플리케이션

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. The active ester of Atto 647N fluorescence dye reacts with amino groups under mild conditions. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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