08951
6-Carboxy-fluorescein diacetate N-succinimidyl ester
for fluorescence, ≥90.0% (HPLC)
동의어(들):
6-Carboxy-di-O-acetylfluorescein N-succinimidyl ester
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모든 사진(1)
About This Item
실험식(Hill 표기법):
C29H19NO11
CAS Number:
Molecular Weight:
557.46
MDL number:
UNSPSC 코드:
12352108
PubChem Substance ID:
NACRES:
NA.32
추천 제품
Grade
for fluorescence
분석
≥90.0% (HPLC)
solubility
DMF: soluble
DMSO: soluble
형광
λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
저장 온도
−20°C
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(=O)ON6C(=O)CCC6=O)c1
InChI
1S/C29H19NO11/c1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
InChI key
JGPOSNWWINVNFV-UHFFFAOYSA-N
애플리케이션
The succinimidyl ester group is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.
기타 정보
Reagent for continuously determining the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
![5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride ≥90% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/271/656/5f185b68-9254-440f-8211-1f4fbe1fee16/640/5f185b68-9254-440f-8211-1f4fbe1fee16.png)
H E Gruber et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 118-123 (2000-08-19)
We have assessed the utility of an intracellular fluorochrome, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), as a tracking label for human intervertebral disc cells in vitro. Although 5 microM provides adequate intracellular labeling for whole cell fluorescent microscopic identification of labeled
P Breeuwer et al.
Applied and environmental microbiology, 62(1), 178-183 (1996-01-01)
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate
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