C0537

5-Carboxyfluorescein

≥99% purity (HPLC), powder

• 동의어(들): 5-FAM
• 실험식(Hill 표기법): C₂₁H₁₂O₇
• CAS Number: 76823-03-5
• Molecular Weight: 376.32
• Beilstein: 57037
• MDL number: MFCD00036874
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24892258
• NACRES: NA.47

속성

• product name: 5-Carboxyfluorescein, ≥99% (HPLC)
• Quality Level: 200
• 분석: ≥99% (HPLC)
• 형태: powder
• solubility: 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: 2-8°C
• SMILES string: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

설명

애플리케이션

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

생화학적/생리학적 작용

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)