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Merck
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Key Documents

69966

Millipore

MRS Broth

suitable for microbiology, NutriSelect® Basic

동의어(들):

Lactobacillus Broth acc. to De Man, Rogosa and Sharpe

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About This Item

UNSPSC 코드:
41171607
NACRES:
NA.74

무균

non-sterile

Quality Level

형태

powder

유통기한

limited shelf life, expiry date on the label

구성

dipotassium hydrogen phosphate, 2 g/L
glucose, 20 g/L
magnesium sulfate monohydrate, 0.2 g/L
manganous sulfate tetrahydrate, 0.05 g/L
meat extract, 8 g/L
peptone, 10 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
yeast extract, 4 g/L

포장

pkg of 500 g

제조업체/상표

NutriSelect® Basic

기술

microbiological culture: suitable

pH

6.2±0.2

응용 분야

agriculture
bioburden testing
environmental
food and beverages

microbiology

적합성

selective by low pH for Bifidobacterium spp.
selective by low pH for Lactobacillus spp.

일반 설명

MRS Broth is used for the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. The medium can be used for confirmatory tests of colonies isolated from MRS Agar. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium, and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species, as well as other secondary bacteria, may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide, and polymyxin. These substances can be used at varying concentrations and combinations, but inevitably a compromise must be reached between selectivity and recovery rate of the organism.

애플리케이션

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

제조 메모

Dissolve 51.25 g in 1 litre distilled water and add 1 ml Tween® 80 (Cat. No. P8074). Boil to dissolve the medium completely. Fill into containers and sterilize by autoclaving at 121°C for 15 minutes. lncubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the culture in a CO2 enriched atmosphere in an anaerobic jar.

각주

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

법적 정보

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

이미 열람한 고객

Slide 1 of 2

1 of 2

L.C. Laleye et al.
Journal of Food Protection, 50, 1009-1009 (1987)
P Laloi et al.
Applied microbiology and biotechnology, 36(2), 196-204 (1991-11-01)
Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 were able to hydrolyse alpha- and beta-caseins. Irrespective of the growth medium used, milk or De Man-Rogosa-Sharpe (MRS) broth, identical patterns of alpha- and beta-casein hydrolytic products, respectively, were visualized by
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes

문서

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

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