추천 제품
Agency
according to ISO 10273:2017
Quality Level
무균
non-sterile
제품 라인
BioChemika
형태
powder
유통기한
limited shelf life, expiry date on the label
구성
agar, 15.0 g/L
esculin, 1 g/L
ferric citrate, 0.5 g/L
meat extract, 3 g/L
meat peptone, 5 g/L
ox-bile, 40 g/L
제조업체/상표
NutriSelect® Plus
기술
microbe id | specific enzyme detection: suitable
최종 pH
6.6±0.2 (25 °C)
응용 분야
environmental
food and beverages
water monitoring
microbiology
적합성
Streptococcus spp.
selective and differential for Enterococcus spp.
애플리케이션
Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.
For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).
제조 메모
Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.
각주
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
법적 정보
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation. Microbiology notes. Pratiksha Pokhrel September 1, 2015 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation2015-10-16T17:40:41+00:00 Bacteriology, Biochemical Test, Diagnosis, Laboratory.
Science (2015)
Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn
Journal of Clinical Microbiology, 1 (5), 440-443 (1975)
R R Facklam et al.
Applied microbiology, 20(2), 245-250 (1970-08-01)
Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for
The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).
A SWAN
Journal of clinical pathology, 7(2), 160-163 (1954-05-01)
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