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Merck
모든 사진(3)

주요 문서

114375

Sigma-Aldrich

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

동의어(들):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

실험식(Hill 표기법):
C21H16Br2O5S
CAS Number:
Molecular Weight:
540.22
Beilstein:
359618
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

제품 라인

BioReagent

Quality Level

분석

≥89.0% (HPLC)

양식

powder

구성

Dye content, 90%

기술

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λmax

419 nm

ε (흡광계수)

≥22000 at 418-424 nm in methanol

적합성

suitable for indicator

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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애플리케이션

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Spectrophotometric determination of serum-albumin

생화학적/생리학적 작용

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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문서 라이브러리 방문

Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
Munzer Kattan et al.
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine, 69(2), 377-380 (2010-11-27)
Polyvinyl chloride (PVC) dyed with bromocresol purple was investigated as a high-dose radiation dosimeter. The absorbance at 417 nm depends linearly on the dose below 50 kGy. The response depends neither on dose rate nor on the irradiation temperature. The
Faizah Mohd Faizul et al.
Journal of photochemistry and photobiology. B, Biology, 90(1), 1-7 (2007-11-21)
The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied

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