추천 제품
무균
sterile; 0.2 μm filtered
Quality Level
형태
solution
포장
pkg of 10 mL (50x)
제조업체/상표
Roche
농도
50 ×
기술
cell culture | hybridoma: suitable
불순물
mycoplasma, tested
100 EU/mL endotoxin (LAL test)
solubility
water: miscible
저장 온도
−20°C
일반 설명
Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.
애플리케이션
Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.
단위 정의
Endotoxin (LAL): 100 EU/ml, mycoplasma tested
물리적 형태
Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.
제조 메모
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
Nutridoma is a trademark of Roche
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Debora Steiner et al.
Nature biotechnology, 28(4), 361-364 (2010-03-31)
Undifferentiated human embryonic stem cells (hESCs) are currently propagated on a relatively small scale as monolayer colonies. Culture of hESCs as floating aggregates is widely used for induction of differentiation into embryoid bodies. Here we show that hESC lines can
Mariana Capurro et al.
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Oliver Sterthaus et al.
Cellular reprogramming, 16(3), 185-195 (2014-05-09)
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four lines. The observed differences were typical of
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