추천 제품
설명
Molecular Formula: C33H39N8O6COOCH3
Quality Level
분석
90% (enzymatic)
형태
powder
분자량
Mr 702.9
포장
pkg of 20 mg
제조업체/상표
Roche
저장 온도
room temp
관련 카테고리
일반 설명
Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.
애플리케이션
Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
품질
Contaminant: <0.5% free 4-nitraniline
서열
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
원리
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
제조 메모
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
Chromozym is a registered trademark of Pentapharm AG, Basel
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Clinical chemistry, 27(4), 586-593 (1981-04-01)
The amidolytic activity of activated kallikrein in plasma can be measured by use of the chromogenic substrate, S-2302 (H-D-Pro-Phe-Arg-pNA). Plasma prekallikrein was activated to kallikrein by exposure to 50 mg/L dextran sulfate in acetone/water (35/65 by vol) at 0 degrees
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