10109045001

Pyruvate Kinase (PK)

from rabbit muscle

• 동의어(들): PK
• 효소 위원회 번호: 2.7.1.40 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 100
• 형태: solution
• 특이 활성도: ~200 units/mg protein (At 25 °C with PEP as the substrate.)
• 포장: pkg of 1 mL (10 mg)
• 제조업체/상표: Roche
• 최적 pH: 7.0-7.5
• 배송 상태: dry ice
• 저장 온도: −20°C (−15°C to −25°C)

설명

일반 설명

Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn²⁺ and phosphoenolpyruvate.

특이성

Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.

애플리케이션

Pyruvate Kinase (PK) has been used as a sample in ATPase Assays.

Pyruvate Kinase (PK) has been used in the kinase assay.

생화학적/생리학적 작용

Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.

품질

Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.

물리적 형태

Solution in 50% glycerol (v/v), pH approximately 6

제조 메모

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available