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Merck
모든 사진(1)

Key Documents

10106399001

Roche

GTP

>90% (HPLC), crystals, pkg of 250 mg

동의어(들):

Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na2

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About This Item

실험식(Hill 표기법):
C10H16N5O14P3 · xNa+ · yH2O
CAS Number:
Molecular Weight:
523.18 (anhydrous free acid basis)
Beilstein:
4113439
MDL number:
UNSPSC 코드:
41106305
PubChem Substance ID:

설명

Disodium salt

Quality Level

분석

>90% (HPLC)

형태

crystals

분자량

Mr 523.2 (GTP)
Mr 567.1 (GTP-Na2)

포장

pkg of 250 mg

제조업체/상표

Roche

저장 조건

avoid repeated freeze/thaw cycles

SMILES string

O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1

InChI key

HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
GTP is supplied as the disodium salt in crystal form.

애플리케이션

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

품질

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O

제조 메모

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

저장 및 안정성

Store at 2 to 8 °C. (Store dry!)

기타 정보

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Lehninger, A.L
Biochemistry, 316-316 (1977)
Marzia Govoni
Bio-protocol, 7(3), e2120-e2120 (2017-02-05)
In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase
Ewa Król et al.
Journal of visualized experiments : JoVE, (81)(81), e50844-e50844 (2013-12-05)
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to form the so-called Z-ring. FtsZ polymerizes into long filaments in the presence of GTP in vitro, and polymerization is regulated by several accessory proteins.
The role of guanosine 5'-triphosphate in the initiation of peptide synthesis. 3. Binding of formylmethionyl-tRNA to ribosomes.
T Ohta et al.
Proceedings of the National Academy of Sciences of the United States of America, 58(4), 1638-1644 (1967-10-01)
The role of guanosine 5'-triphosphate in polypeptide chain elongation.
Y Kaziro
Biochimica et biophysica acta, 505(1), 95-127 (1978-09-21)

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