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Merck
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Key Documents

SCT134

Sigma-Aldrich

BioTracker 510 Green C18(FM3-25) Synaptic Dye

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

동의어(들):

Live cell imaging probe

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.47

기술

cell based assay: suitable

검출 방법

fluorometric

배송 상태

ambient

일반 설명

BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.

BioTracker 510 Green C18(FM3-25) Synaptic Dye has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.

Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm

Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nm

애플리케이션

Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

품질


Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm

Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nm

물리적 형태

Lyophilized

저장 및 안정성

Store BioTracker 510 Green C18(FM3-25) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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