SCT043

BioTracker TP-HOCL1 Live Cell Dye

Live cell imaging dye for hypochlorus acid (HOCL) used to detect phagocytosis, inflammation and cellular apoptosis.

• 동의어(들): Live cell imaging probe
• UNSPSC 코드: 12352207
• NACRES: NA.47

속성

• 분석: ≥98% (H-NMR); ≥98% (HPLC); ≥98% (LC/MS); ≥98% (elemental analysis)
• 기술: cell based assay: suitable
• 검출 방법: fluorometric
• 배송 상태: ambient

설명

일반 설명

Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

The BioTracker TP-HOCL1 dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. 2- mercaptoethanol and 1, 2-ethanedithiol were employed to protect the ketone of acedan in the design of HOCl probes. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement

Spectral Properties
Absorbance: 375nm
Emission: 500nm

References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.

애플리케이션

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

품질

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 258.36 g/mol

물리적 형태

Lyophilized

저장 및 안정성

Store BioTracker TP-HOCL1 Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable