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Merck
모든 사진(1)

주요 문서

SCM304

Sigma-Aldrich

3dGRO® Human Colon Organoid Expansion Medium

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

동의어(들):

Colon Organoid Culture Media, Intestinal Organoid Culture Media

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

형태

liquid

포장

pkg of 50 mL

기술

cell culture | stem cell: suitable

몰랄삼투압농도

350-375 mOsm

응용 분야

cell analysis

일반 설명

3dGRO® Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO® Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.

애플리케이션

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

Research Category:
  • Cell Culture
  • Stem Cell Research

품질

Appearance (Color): Clear/No Particlulates (Red Liquid)
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.

저장 및 안정성

Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.

법적 정보

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

프로토콜

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.