SCCAR10064

Assay Ready LX-2 Human Hepatic Stellate Cell Line

Human

• 동의어(들): assay ready hepatic stellate cells, assay ready hepatocytes, hepatic fibrosis cell line, hepatic stellate cell line, hepatocyte cell line
• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• product name: Assay Ready LX-2 Human Hepatic Stellate Cell Line,
• 생물학적 소스: human
• Quality Level: 100
• 포장: vial of 1 vial
• 제조업체/상표: Millipore
• 기술: cell culture | stem cell: suitable
• 배송 상태: liquid nitrogen
• 저장 온도: −196°C

설명

일반 설명

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

특징 및 장점

LX-2 cells retain key features of human hepatic stellate cells including cytokine signaling, retinoid metabolism, and fibrogenesis. **SCCAR10064 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.

표적 설명

This product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see <IS7_PDP/PFPLink_from_Module_content target="https://www.sigmaaldrich.com/US/en/product/mm/scc064">SCC064</IS7_PDP/PFPLink_from_Module_content>.


Hepatic stellate cells are an essential cell phenotype responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for the development of therapeutics.

The LX-2 cell line was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early-passage cells in low serum media conditions.

저장 및 안정성

LX-2 cells should be stored in liquid nitrogen.

기타 정보

This product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable