SCC234

YUMM2.2 Mouse Melanoma Cell Line

Mouse

• 동의어(들): Yale University Mouse Melanoma 2.2 cell line, Yale University Mouse Melanoma cell line
• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• product name: YUMM2.2 Mouse Melanoma Cell Line,
• 생물학적 소스: mouse
• Quality Level: 100
• 포장: vial of ≥1x10^6 vial
• 제조업체/상표: Millipore
• 기술: cell culture | mammalian: suitable
• 배송 상태: liquid nitrogen
• 저장 온도: −196°C

설명

일반 설명

The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM2.2 cells are syngeneic with the immunocompetent C57Bl/6 mouse background.

애플리케이션

• Each vial contains > 1X10⁶ viable cells.
• YUMM2.2 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from rat, chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

특징 및 장점

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2.2 harbors a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis and inactivates wt Pten and Cdkn2a.

표적 설명

The hope for immune-based therapies in cancer and recent progress in successful application of these approaches has focused research on the necessity for immunocompetent models to assess immune system responses to cancer cells. Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.The YUMM2.2 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.SourceThe YUMM2.2 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model had been introduced via backcrossing. References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

저장 및 안정성

YUMM2.2 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.

기타 정보

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable