추천 제품
product name
CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line, CFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways.
생물학적 소스
human
기술
cell based assay: suitable
cell culture | mammalian: suitable
배송 상태
ambient
일반 설명
CFTE29o- is a human CF tracheal epithelial cell line, derived from a 21-yr old male CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . The cell line express keratin, indicating an epithelial cell origin, along with a calcium-dependent cell adhesion protein, cellCAM 120/80 , and ZO-1 indicating the ability to form tight junctions. CFTE29o- displays ion transport properties characteristic of cystic fibrosis such as defective cAMP-dependent chloride transport. Upon treatment with the calcium ionophore, ionomycin, CFTE29o- secretes chloride, albeit at reduced levels relative to normal cells. The cells express CFTR mRNA, and small amounts of CFTR protein as assessed by Western blot hybridization . Karyotype analysis indicates that 70% of the cells contain two copies of chromosome 7 .
CFTE29o- is a useful model for cystic fibrosis and for studying chloride ion transport and ion transport in human airways. The cells are useful for testing candidate chemotherapeutic drugs for their potential as treatments for ameliorating CF pathologies.
세포주 설명
애플리케이션
품질
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
저장 및 안정성
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.