PS1019

PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem^®

• 동의어(들): Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr ^183/185), Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr^183/185)
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: mouse, rat, human
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 동형: IgG
• 배송 상태: wet ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: phosphorylation (pThr183/pTyr185)
• 유전자 정보: human ... MAPK8(5599)

설명

일반 설명

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵ in uv-treated HEK293 cells.

This PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

면역원

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

애플리케이션

Immunoblotting (1:1000)

경고

Toxicity: Standard Handling (A)

물리적 형태

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

재구성

Following initial thaw, aliquot and freeze (-20°C).

분석 메모

Positive Control
UV-treated HEK293 cells or sorbitol-treated PC12 cells

기타 정보

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol.21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.
Tong, T., et al. 2001. Exp. Cell Res.269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.
Basu, S., et al. 1998. Oncogene17, 3277.

The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1